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KMID : 1123920090230020457
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Korean Journal of Oriental Physiology and Pathology
2009 Volume.23 No. 2 p.457 ~ p.463
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Effects of Seonpye-tang on OVA-Induced Asthmatic Mice
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Lee Ju-Guan
Park Yang-Chun
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Abstract
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This study aimed to evaluate the anti-asthmatic effects of Seonpye-tang (SPT) using OVA-induced asthmatic mice model. Scavenging activity of SPT on DPPH free radical and SOD-like activity of SPT were measured at final concentration 62.5, 125, 250, 500 (), RBL-2H3 cells were treated with DNP IgE for 24hr, and treated with SPT (1, 10, 100 ) for 1hr, followed by treatment with DNP-HSA for 1hr at . The level of IL-4 and TNF- were measured by ELISA. Asthmatic mice model was conducted by repeated challenge of OVA using C57BL/6 mice. Each group was treated with distilled water, SPT (400 mg/kg and 200 mg/kg) extract or cyclosporin A (10 mg/kg) for the later 8 weeks. Immune cells subpopulation, eotaxin, IL-5 and TNF- in BALF were analyzed. SPT dose-dependently increased Scavenging activity on DPPH free radical and SOD-like activity. SPT significantly ameliorated the increase of total cells number and eosinophil including of immune cell subpopulation of , , , and cells in BALF comparing to control group. Eotaxin and IL-5 level in BALF were significantly decreased by SPT. These results strongly suggest that SPT would be a effective candidate for herbal-originated anti-asthmatic drug. However, this drug should be further studied for characterization of the accurate action and underlying mechanisms using variant disease model in the future.
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KEYWORD
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Seonpye-tang, asthma, antioxidative effect, immune cell, cytokine
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